Ανίχνευση εσφαλμένης επισήμανσης σε αλιεύματα που φέρουν την εμπορική ονομασία «ΓΑΛΕΟΣ», με τη χρήση μιτοχονδριακών DNA δεικτών (Master thesis)
Παζαρτζή, Θεοδοσία/ Σιαπεροπούλου, Στυλιανή
In the trade of many species, food processing usually causes the loss of any morphological diagnostic features of the species, enhancing the possibility of fraudulent substitution and mislabeling. Lack of morphological features due to food processing is common in elasmobranchs, as all external traits are discarded (e.g. skin, head and ﬁns), and the body part is processed into ﬁlets. Such methods increase the chances of species substitution with cheaper alternatives or endangered species, leaving consumers unprotected from unfair or deceptive practices. DNA Barcoding has become the most commonly applied approach for detection of mislabeled seafood products and species substitution, because morphological identiﬁcation is impossible, due to the processing of the ﬁsh. According to the Greek food and safety legislation requirements (Oﬃcial Government Gazette 475/ Issue B’/27-3-2015, No. 1750/32219 under EU 1379/2013), only the Mustelus spp. products (Mustelus mustelus, M. punctulatus and M. asterias) can be sold under the commercial term "galeos". A total of 74 samples sold under the name galeos, were collected from 24 ﬁshmongers and open markets located in four diﬀerent cities of Greece (Athens, Thessaloniki, Kavala and Komotini), between March 2016 and May 2018. Genomic DNA was extracted from the sample tissue of fish, and the quantity and quality of the DNA were visualized on an agarose gel, with the use of ultraviolet radiation. Two diﬀerent mtDNA genes (16S rRNA and COI) were selected for the analysis of each sample, and all PCR products were electrophorsed on agarose gel. The PCR products were purified and sequenced in an ABI automated analyzer. All produced sequences were checked and compared with those available in GenBank and BOLD databases, for species identification. Across the study, an extremely high level of mislabeling was identiﬁed (54.8%), as only 33 from 74 samples were classified to the Mustelus spp. species. It is worth mentioning that over half of products originate from species belonging to the IUCN red list (53.4%), and from species with prohibitions on landings by international and national legislation (57.6%). Τhe substitution of large-growing species, with little morphological resemblance to Mustelus spp., likely demonstrates deliberate substitution
|Institution and School/Department of submitter:||Σχολή Τεχνολογίας Τροφίμων / Τμήμα Επιστήμης και Τεχνολογίας τριφίμων|
|Keywords:||Αλιεύματα;Εμπορική ονομασία;Γονίδια;Γαλέος;PCR(Polymerase Chain Reaction);DNA ( Deoxyribonucleic acid)|
|Description:||Μεταπτυχιακή εργασία = Σχολή Τεχνολογίας Γεωπονίας & Τεχνολογίας Τροφίμων & Διατροφής –=Τμήμα Τεχνολογίας Τροφίμων, 2019 (α/α 11479)|
|Appears in Collections:||Μεταπτυχιακές Διατριβές|
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|Παζαρτζή- Σιαπεροπούλου.pdf||Μεταπτυχιακή εργασία||7.63 MB||Adobe PDF||View/Open|
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