Molecular identification and anti-pathogenic activities of putative probiotic bacteria isolated from faeces of healthy elderly individuals
Tuohy, Kieran/ Gibson, Glenn/ Rastall, Robert/ Manderson, Kirstie/ Fava, Francesca/ Likotrafiti, Eleni/ Λυκοτραφίτη, Ελένη
|Institution and School/Department of submitter:||ΤΕΙ Θεσσαλονίκης|
|Publisher:||Taylor & Francis|
|Citation:||Journal: Microbial Ecology in Health and Disease, vol. 16, no. 2-3, 2004|
Likotrafiti, E., Manderson, K., Fava, F. & Tuohy, K. (2004). Molecular identification and anti-pathogenic activities of putative probiotic bacteria isolated from faeces of healthy elderly individuals. Microbial Ecology in Health and Disease. 16(2-3):105-112.
|Abstract:||One hundred and nine lactic acid bacterial strains (56 bifidobacteria-like and 53 lactobacilli-like) were isolated from faecal samples donated by healthy elderly individuals (>65 years old). Isolates were identified to species level by phenotypic analysis (by API) and by 16S rDNA sequencing. Eleven species of Lactobacillus and six species of Bifidobacterium were identified. The most frequently isolated lactobacillus was L. fermentum and the most frequently isolated bifidobacterium was closely related to B. infantis by 16S rDNA sequence alignment. The isolates were characterized for their antimicrobial activity against Clostridium difficile, enteropathogenic Escherichia coli (EPEC), verocytotoxigenic E. coli (VTEC) and Campylobacter jejuni. The lactobacilli displayed variations in their antimicrobial activity with few strains showing inhibitory activity against all pathogens. The bifidobacteria displayed higher levels of inhibitory activity against C. jejuni and Cl. difficile than against the E. coli strains. Keywords: Lactobacillus, Bifidobacterium, elderly, gastrointestinal microbiota, inhibition, Clostridium difficile, enteropathogenic Escherichia coli (EPEC), verocytotoxigenic E. coli (VTEC), Campylobacter jejuni.|
|Description:||Δημοσιεύσεις μελών--ΣΤΕΓΤΕΤΡΟΔ--Τμήμα Τεχνολογίας Τροφίμων,2004|
|Appears in Collections:||Δημοσιεύσεις σε Περιοδικά|
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