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Introduction Diabetes Mellitus (DM) is a major health problem for modern societies. Changing in lifestyle, a variety of predisposing factors and the adoption of an not properly structured diet contribute to the increase of DM. Healthcare professionals are, all the more, called upon to provide care for people with DM. The aim of the present study was to investigate the knowledge, the attitudes and behavior of a sample of healthcare professionals regarding Diabetes Mellitus. Methods: The study population was a convience sample consisting of healthcare professionals, nurses and doctors, employed in Inter-Balkan Medical Center of Thessaloniki (n=100). Respondents' demographic characteristics were recorded and with validated and reliable questionnaires, their emotions (Semantic Differential Scale for Diabetes, DSDS) and their attitude towards diabetes mellitus (Diabetes Behavior Study, DAS), as well. Results: The sample were 41(± 12.03) years old, with men accounting for 54.1% of them. The total sample had an experience of 17,20(±10.86) years, consisting mainly of physicians (39.8%) and nurses (19.4%). All healthcare professionals appeared to have rather positive feelings toward DM (4.27 ±1.71 on DSDS). Participants also were found to have a need for special training on DM (1.64±0.45 at DAS), with a rather neutral attitude towards disease severity (2.69 ±0.63) and the value of strict glycemic control (2.45 ±0.49). Finally, it was found that for each year of age passed there was a more positive perception of the severity of the disease, while for each year of employement a more positive perception of psychosomatic effects of DM was identified. Conclusions: Training of health personnel in counseling and patient communication is considered necessary, as well-organized continuing education interventions can facilitate the adoption of best practices in disease management, self-care and compliance with the therapeutic approach.