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The present study was conducted with a sample of 10 participants, 5 women and 5 men from 23 to 27 years of age in different types of training, in order to investigate whether there is a relationship between protein intake and muscle mass. The survey was conducted during a month pediod, from February 1st to March 1st 2017, with sportsmen and women on the island of Lesbos. The data were collected by means of questionnaires, counting, weighing and other recording methods and then processed with the Statistical Package for Social Sciences (SPSS), version 22. Taking into account the small size of the sample and therefore based on numerical and percentage correlations, according to the results there is an indication that there is a relationship between protein intake and muscle mass. Athletes consuming more protein and fewer carbohydrates had higher muscle mass and lower fatty tissue than others. Frequency of training has shown similar results. Those with a higher frequency of workouts had lower fat tissue rates and higher muscle mass ratios, respectively. Also those with resistance training had a higher muscle mass than those who did another type of training such as swimming and running. Men had more muscle mass than women. Finally, the investigation showed that people who used supplements had higher muscle mass than those who did not take supplements.