Eureka! Institutional Repository

Tigecycline is a glycylcycline expanded broad-spectrum antibiotic which exhibits robust activity against bacterial which are resistant to other antibiotic classes. Tigecycline has been evaluated as monotherapy for several infections, such as intraventricular and leather infections, due to its microbiologic, pharmacokinetic and pharmacodynamic properties. In this project has been investigated the pharmacokinetic profile of tigecycline which describes the stages from the time the drug enters the body until its final excretion. Specifically, has been determined the concentration of tigecycline in blood serum of rats and in plasma of adults patients with the method of Liquid Chromatography-Mass Spectrometry (LC-MS/MS). The results were obtained after sampling conducted at specific intervals over 24 hours, leading to the conclusion that the concentration of tigecycline both in serum of rats and in plasma of patients displays a maximum concentration during the first half hour after administration of the drug, while in then a gradual reduction. This is due to the approach followed tigecycline within the body which is depended on the enzymatic and metabolic processes that lead to absorption, tissue distribution, metabolism and eventually excretion. Furthermore, has been developed and validated a new UPLC-HILIC tandem Ms method (Hydrophilic Liquid Chromatography) for the determination of 21 amino acids in flour of emmer wheat. For the method development and final sample preparation, was used many hydrolysis tests and various tests for the selection of the appropriate mobile phase. The optimization of the spectrometer detector was made with the use of automated operation of SRM (Single Reaction Monitoring), while for the sample ionisation was selected the method of Electrospray positive ionisation mode (ESI). The method has proved suitable for compounds such amino acids, as it can reduce the disadvantages of other analytical methods.